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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
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Time Radically Alters Ex Situ Evidentiary Soil 16S Bacterial Profiles Produced Via Next-Generation Sequencing,.
Alyssa J Badgley1, Ellen M Jesmok1, David R Foran2
1Forensic Science Program, School of Criminal Justice, Michigan State University, 655 Auditorium Road, 560 Baker Hall, East Lansing, MI, 48824.
Journal of Forensic Sciences
|February 22, 2018
Summary
Soil bacterial profiles on aged evidence show consistent changes over time. This research enhances the use of soil microbial analysis in forensic science investigations.
Area of Science:
- Forensic microbiology
- Environmental microbiology
- Molecular ecology
Background:
- Soil bacterial profiling shows forensic potential.
- Temporal changes in soil microbial communities on aged evidence are understudied.
Purpose of the Study:
- To investigate temporal bacterial profile shifts in soil aged on evidence.
- To assess the utility of these shifts for forensic source attribution.
Main Methods:
- Soil samples from diverse habitats were aged on evidence items.
- Bacterial profiles were generated using 16S rRNA gene sequencing.
- Temporal changes were visualized, and supervised classification was employed.
Main Results:
- Ex situ soils exhibited consistent taxonomic changes with aging.
- Bacterial profiles drifted in multidimensional space but remained habitat-specific.
- 95% of evidence profiles were correctly classified to their source habitat.
Conclusions:
- Temporal bacterial dynamics in aged soils are predictable.
- Understanding these changes improves the forensic application of soil microbial data.
- Evidence type and age influence classification accuracy.
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