Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.9K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.9K
Electronic Distance Measuring Instruments01:30

Electronic Distance Measuring Instruments

543
Electronic Distance Measuring Instruments (EDMs) are essential tools in modern surveying, offering precise distance measurements by emitting electromagnetic signals and calculating the time required for these signals to travel to a target and return. Two primary types of signals are used in EDMs — light waves and microwaves — each suited to specific environmental and distance requirements. Light-wave-based EDMs utilize either infrared or laser light, providing high accuracy over...
543
Protein-protein Interfaces02:04

Protein-protein Interfaces

14.8K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.8K
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

4.5K
4.5K
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

17.9K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
17.9K
Electron Affinity03:07

Electron Affinity

43.7K
The electron affinity (EA) is the energy change for adding an electron to a gaseous atom to form an anion (negative ion).
43.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Special issue: Quantitative and precise measurements in conventional (scanning) electron microscopy and electron tomography.

Micron (Oxford, England : 1993)·2026
Same author

Beam commissioning of a new compact scanned proton therapy system with four different dose calculation algorithms.

Journal of applied clinical medical physics·2025
Same author

Momentum-dispersion calibration and measurement of a surface layer dielectric constant using momentum-resolved electron energy-loss spectroscopy in the optical region.

Micron (Oxford, England : 1993)·2025
Same author

Superficial Temporal Artery-Based ReMAP: A Case Report Series in Head and Neck Cancer Intra-Arterial Chemotherapy.

Head & neck·2025
Same author

A comparison between hollow cone illumination and wavelet transform methods for chromatin fiber orientation measurement.

Micron (Oxford, England : 1993)·2025
Same author

Female and preserved platelet count subgroups of myelodysplastic syndrome patients benefit from standard-dose azacitidine.

Cancer reports (Hoboken, N.J.)·2023

Related Experiment Video

Updated: Feb 14, 2026

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
09:53

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows

Published on: September 13, 2021

7.7K

Evaluation of electron tomography reconstruction methods for interface roughness measurement.

Misa Hayashida1, Shinichi Ogawa2, Marek Malac1,3

  • 1Nanotechnology Research Centre, National Research Council, Edmonton, T6G 2M9, Canada.

Microscopy Research and Technique
|February 22, 2018
PubMed
Summary

Simultaneous iterative reconstruction technique (SIRT) is best for buried interface roughness measurements. Optimal tilt angles for accurate roughness measurement range from 3° to 5°.

Keywords:
buried interface roughnessdose fractionation theoremelectron tomographyradiation damagesemiconductor devices

More Related Videos

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
11:33

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

11.4K
Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

5.9K

Related Experiment Videos

Last Updated: Feb 14, 2026

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
09:53

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows

Published on: September 13, 2021

7.7K
Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
11:33

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

11.4K
Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

5.9K

Area of Science:

  • Materials Science
  • Electron Microscopy
  • Image Reconstruction

Background:

  • Accurate measurement of buried interface roughness is crucial in materials science.
  • Traditional reconstruction methods may introduce artifacts affecting roughness quantification.
  • Understanding the impact of electron dose and tilt angle is vital for reliable data.

Purpose of the Study:

  • To compare the suitability of different reconstruction algorithms for buried interface roughness measurements.
  • To investigate the influence of total electron dose and dose fractionation on roughness quantification.
  • To determine optimal acquisition parameters for precise roughness measurements.

Main Methods:

  • Evaluation of Simultaneous Iterative Reconstruction Technique (SIRT), Filtered Back Projection (FBP), and Simultaneous Algebraic Reconstruction Technique (SART).
  • Analysis of roughness measurements under varying total electron doses.
  • Assessment of the dose fractionation theorem by varying the number of images (tilt increments).

Main Results:

  • SIRT demonstrated superior performance for reconstruction compared to FBP and SART.
  • Roughness measurements were sensitive to the distribution of electron dose across the tilt series.
  • An optimal tilt angle range of 3° to 5° was identified for accurate roughness quantification.

Conclusions:

  • SIRT is the recommended reconstruction method for buried interface roughness analysis.
  • Careful consideration of electron dose distribution and tilt angle is necessary for reliable measurements.
  • The study provides guidelines for optimizing electron tomography acquisition for interface roughness characterization.