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Laparoscopic Extracorporeal Knot-Tying for Uterine Vessel Occlusion during Hysterectomy with Cervical Cerclage in Large Uteri
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Laparoscopic Extracorporeal Knot-Tying for Uterine Vessel Occlusion during Hysterectomy with Cervical Cerclage in Large Uteri

Published on: September 12, 2025

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Differential Expression of MicroRNAs in Uterine Cervical Cancer and Its Implications in Carcinogenesis; An

Veena B Nair, V G Manasa, M S Sinto

    International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society
    |February 22, 2018
    PubMed
    Abstract

    Insights

    This study reveals that high-risk human papillomavirus (HPV) triggers similar cervical cancer development mechanisms regardless of HPV type. MicroRNA (miRNA) profiling identified key miRNA signatures involved in cancer pathways, offering insights into cervical cancer's molecular origins.

    Area of Science:

    • Oncology
    • Molecular Biology
    • Virology

    Background:

    • Cervical cancer is a significant health issue, particularly in developing nations.
    • MicroRNAs (miRNAs) play crucial roles in cellular processes and cancer development.
    • Understanding miRNA involvement in cervical carcinogenesis is vital for targeted therapies.

    Purpose of the Study:

    • To profile miRNA expression patterns in cervical cancer.
    • To identify target genes of deregulated miRNAs.
    • To elucidate the role of miRNAs in cervical carcinogenesis, especially concerning human papillomavirus (HPV) infection.

    Main Methods:

    • Human papillomavirus (HPV) infection assessed using heminested PCR and DNA sequencing.
    • miRNA profiling conducted via next-generation sequencing and miRNA microarray.
    • miRNA signature validation using quantitative real-time PCR and in silico analyses.

    Main Results:

    • High prevalence of HPV type 16 (93%) in cervical cancer tissues.
    • Distinct miRNA expression patterns observed concerning HPV positivity.
    • Identified miRNA signatures primarily involved in PI3K-Akt and mTOR signaling pathways.

    Conclusions:

    • High-risk HPV infection leads to similar carcinogenic mechanisms irrespective of the specific HPV type.
    • Elucidation of cervical cancer miRNA signatures and their target genes provides deeper insight into molecular mechanisms.
    • Findings contribute to understanding cervical cancer development and potential therapeutic targets.

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