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Related Concept Videos

Transcription Factors02:16

Transcription Factors

82.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.9K
Transcription01:10

Transcription

157.1K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
157.1K
Master Transcription Regulators02:23

Master Transcription Regulators

7.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.8K
Transcription Elongation Factors02:35

Transcription Elongation Factors

14.1K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
14.1K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

6.1K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
6.1K
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

11.1K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
11.1K

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Related Experiment Video

Updated: Feb 14, 2026

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach
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Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach

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A LIN28B Tumor-Specific Transcript in Cancer.

Weijie Guo1, Zhixiang Hu2, Yichao Bao3

  • 1Fudan University Shanghai Cancer Center and Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China; Markey Cancer Center, College of Medicine, University of Kentucky, Lexington, KY 40506, USA.

Cell Reports
|February 22, 2018
PubMed
Summary

A novel cancer transcript, LIN28B-TST, is specifically expressed in hepatocellular carcinoma (HCC) and other cancers, correlating with poor prognosis. This LIN28B variant drives cancer cell proliferation and tumorigenesis.

Keywords:
LIN28Boncogenetumor-specific transcript

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Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Genomics

Background:

  • The cancer transcriptome exhibits unique transcripts.
  • LIN28B is implicated in various cancers.

Purpose of the Study:

  • To identify and characterize novel cancer-specific transcripts.
  • To investigate the role of LIN28B variants in hepatocellular carcinoma (HCC).

Main Methods:

  • Transcriptome analysis to identify LIN28B-TST.
  • Promoter analysis to identify regulatory elements (NFYA).
  • Functional assays to assess LIN28B-TST's role in proliferation and tumorigenesis.

Main Results:

  • LIN28B-TST is specifically expressed in HCC and other cancers.
  • LIN28B-TST expression correlates with poor prognosis in HCC patients.
  • LIN28B-TST initiates from a de novo promoter regulated by NFYA and requires demethylation.

Conclusions:

  • LIN28B-TST is a novel oncogenic isoform critical for cancer progression.
  • LIN28B-TST represents a potential biomarker and therapeutic target in cancer.
  • Demethylation and NFYA regulation are key to LIN28B-TST activation in cancer.