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Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
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Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

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Human adipose-derived stem cells inhibit bioactivity of keloid fibroblasts

Xiuxia Wang1, Yan Ma2, Zhen Gao3

  • 1Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhi Zao Ju Road, Shanghai, 200011, China.

Stem Cell Research & Therapy
|February 23, 2018
PubMed
Abstract

Insights

Human adipose-derived stem cells (ADSCs) show promise in treating keloids. ADSC-conditioned medium significantly reduced keloid fibroblast activity and suppressed cell proliferation, suggesting a potential new therapy for this fibrotic disorder.

Area of Science:

  • Regenerative Medicine
  • Fibrosis Research
  • Stem Cell Therapy

Background:

  • Keloids are fibroproliferative disorders resulting from an exaggerated wound healing response.
  • Current treatments for keloids lack definitive efficacy.
  • Human adipose-derived stem cells (ADSCs) offer potential therapeutic benefits for fibrotic conditions.

Purpose of the Study:

  • To investigate the potential of human ADSCs in treating keloids.
  • To evaluate the effects of ADSC-conditioned medium (ADSC-CM) on keloid fibroblast bioactivity.

Main Methods:

  • Keloid fibroblasts were cultured in ADSC-CM.
  • Gene and protein expression, cell cycle, and proliferation were analyzed using RT-PCR and Western blotting.
  • Ex vivo keloid explants were used to assess the impact of ADSC-CM on CD31+ and CD34+ vessel expression.

Main Results:

  • ADSC-CM significantly attenuated extracellular matrix-related gene and protein expression in keloid fibroblasts.
  • Cell proliferation of keloid fibroblasts was markedly suppressed.
  • A significant reduction in CD31+ (55%) and CD34+ (57%) vessels was observed in ex vivo keloid explants treated with ADSC-CM.

Conclusions:

  • Human ADSC-CM demonstrated significant inhibitory effects on keloid fibroblast bioactivities.
  • ADSC-CM presents a promising therapeutic avenue for managing keloid formation and progression.
Keywords:
Adipose-derived stem cellsConditioned mediumKeloid fibroblasts

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