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Updated: Feb 14, 2026
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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
Published on: June 15, 2016
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Endothelial TFEB (Transcription Factor EB) Positively Regulates Postischemic Angiogenesis
Yanbo Fan1, Haocheng Lu2, Wenying Liang2
1From the Department of Internal Medicine, Cardiovascular Center, University of Michigan Medical Center, Ann Arbor. yanbo@med.umich.edu echenum@umich.edu.
Circulation Research
|February 23, 2018
Summary
Transcription factor EB (TFEB) promotes blood vessel formation (angiogenesis) by activating AMPK signaling and autophagy. This discovery highlights TFEB as a potential therapeutic target for ischemic vascular diseases.
Area of Science:
- Molecular Biology
- Vascular Biology
- Cell Biology
Background:
- Postischemic angiogenesis is crucial for tissue repair after ischemia but its regulatory mechanisms are not fully understood.
- Transcription factor EB (TFEB) is a key regulator of autophagy and lysosome biogenesis, but its role in vascular disease remains unclear.
Purpose of the Study:
- To investigate the role of endothelial TFEB in postischemic angiogenesis.
- To elucidate the molecular mechanisms by which TFEB regulates angiogenesis.
Main Methods:
- Utilized primary human endothelial cells (ECs) and EC-specific TFEB transgenic and knockout mouse models.
- Induced hindlimb ischemia in mice to assess blood flow recovery and capillary density.
- Performed in vitro assays including aortic ring culture, EC tube formation, migration, and survival assays.
- Investigated the involvement of AMPKα signaling and autophagy pathways.
Main Results:
- Endothelial TFEB overexpression in mice improved blood perfusion and capillary density post-ischemia.
- TFEB deficiency in ECs attenuated blood flow recovery and impaired vessel sprouting.
- TFEB promoted EC tube formation, migration, and survival in vitro.
- TFEB activated AMPKα signaling and upregulated autophagy, which were essential for its pro-angiogenic effects.
- TFEB-dependent upregulation of MCOLN1 mediated TFEB's effects on AMPKα and EC function.
Conclusions:
- TFEB acts as a positive regulator of angiogenesis in endothelial cells.
- TFEB exerts its pro-angiogenic effects by activating the AMPKα pathway and promoting autophagy.
- TFEB represents a novel molecular target for treating ischemic vascular diseases.
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