Related Experiment Video
Updated: Feb 14, 2026
09:14
Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution
Published on: June 14, 2024
1.8K
Embryonic lethality in mice lacking Trim59 due to impaired gastrulation development
Xiaomin Su1,2,3, Chenglei Wu1,2, Xiaoying Ye4
1Department of Immunology Nankai University School of Medicine, Nankai University, Tianjin, China.
Cell Death & Disease
|February 23, 2018
Summary
Trim59 is essential for early embryonic development, regulating germ layer formation and F-actin assembly. Its absence causes developmental delays and defects from the blastocyst to gastrula stages.
Area of Science:
- Developmental Biology
- Cell Biology
- Molecular Biology
Background:
- TRIM family proteins are involved in various biological processes, including differentiation and development.
- The specific role of Trim59 in early embryogenesis requires further elucidation.
Purpose of the Study:
- To investigate the function of Trim59 in early embryonic development.
- To understand the molecular mechanisms by which Trim59 regulates embryonic development.
Main Methods:
- Generation and analysis of Trim59 knockout (Trim59-/-) mouse embryos.
- Gene expression analysis of primary germ layer formation-associated genes.
- Investigation of Trim59 interactions with cytoskeletal proteins and its role in F-actin polymerization.
Main Results:
- Trim59 deficiency leads to delayed embryonic development, characterized by empty yolk sacs and embryonic lethality beyond E9.5.
- Trim59-/- embryos exhibit defects in primary germ layer formation and reduced expression of key developmental genes.
- Trim59 deficiency disrupts F-actin polymerization in epiblast cells, impacting inner cell mass differentiation.
Conclusions:
- Trim59 is a critical regulator of early embryonic development from the blastocyst to gastrula stages.
- Trim59 modulates F-actin assembly through WASH K63-linked ubiquitination, essential for proper development.
- These findings highlight Trim59's crucial role in cytoskeletal dynamics during embryogenesis.
Related Concept Videos
01:56Gastrulation
67.5K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
67.5K
02:41Lethal Alleles
18.2K
Agouti: A Lethal Allele
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
18.2K
02:33Seed Structure and Early Development of the Sporophyte
31.3K
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.
31.3K
00:58Embryonic Stem Cells
32.7K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.7K
00:57Embryonic Stem Cells
5.2K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
5.2K
01:17Factors Affecting Renal Clearance: Renal Impairment
485
Renal dysfunction significantly impairs the renal clearance of drugs, leading to potential complications in drug therapy. Renal failure, which can be caused by various factors, poses a significant challenge in the elimination of drugs from the body.
One condition associated with renal failure is uremia. Uremia is characterized by impaired glomerular filtration and fluid accumulation in the body. This condition hinders the renal clearance of drugs, resulting in drug accumulation and potential...
One condition associated with renal failure is uremia. Uremia is characterized by impaired glomerular filtration and fluid accumulation in the body. This condition hinders the renal clearance of drugs, resulting in drug accumulation and potential...
485