Related Experiment Video
Updated: Feb 14, 2026

Orthotopic Mouse Model of Colorectal Cancer
Published on: December 4, 2007
In vitro and in vivo Limiting Dilution Assay for Colorectal Cancer
1Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada.
Abstract:
The in vitro limiting dilution assay is used to determine the colorectal cancer initiating cell (CC-IC) frequency of a CC-IC enriched suspension culture, grown in growth factor enriched serum free media. The in vivo limiting dilution assay is used to determine the colorectal cancer initiating cell frequency of a primary colorectal cancer sample or an established suspension cell line using immunocompromised murine xenograft models. In vitro and vivo limiting dilution assays (LDAs) can be used to determine the effect of a specific treatment or genetic knockdown strategy on the initiating cell frequency of a population of CC-ICs or colorectal cancer sample, respectively.
More Related Videos
Related Concept Videos
Solution Concentration and Dilution
Limiting Reactant
Formation of Dilute Urine
Filtrate Osmolarity in the PCT
Initially, as the filtrate passes through the proximal convoluted tubule (PCT), its...
The Number e as a Limit
Equivalence: In Vitro and In Vivo Bioequivalence
Types of Limits I

