Characterization of RB1 Deletions in Interphase and Metaphase by Molecular Cytogenetics Exemplified in Chronic Lymphatic Leukemia
Related Experiment Video
Updated: Feb 14, 2026

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation
Published on: November 26, 2018
1Jena University Hospital, Institute of Human Genetics, Friedrich Schiller University, Jena, Germany. Thomas.Liehr@med.uni-jena.de.
Abstract:
In chronic lymphatic leukemia (CLL), detection and characterization of prognostic relevant chromosomal alterations is optimally done by interphase-fluorescence in situ hybridization (iFISH). Interphase nuclei derived from blood smears, bone marrow smears or from cultivated and conventionally prepared blood or bone marrow cells can be used. In CLL heterozygous or even homozygous deletion of RB1 can be found. Interestingly an iFISH diagnostic result with RB1 deletion as sole aberration is indicative for a favorable course of the disease. Here we describe the best way how to detect RB1 deletion in CLL.
Related Concept Videos
Interphase
Interphase
Phases of Interphase
Following each period of mitosis and cytokinesis, eukaryotic cells enter interphase, during which they grow and replicate...
Development of the Lymphatic System
The first lymph sacs to form are the paired jugular lymph sacs located at the junction of the internal jugular and subclavian veins. From these sacs, lymphatic capillary plexuses extend to the thorax, upper limbs, neck, and head, eventually forming lymphatic vessels. Each jugular lymph sac maintains a...
Functions of the Lymphatic and Immune System
The primary lymphoid organs, including the bone marrow and the thymus, serve as the maturation sites for lymphocytes. Secondary lymphoid organs, like the mucosa-associated lymphoid tissue, activate these lymphocytes and serve as...
Introduction to Lymphatic and Immune System
The immune responses can be categorized into two types: innate and adaptive. Innate immunity comprises nonspecific defenses we are born...
Molecular Models

