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Related Experiment Video

Updated: Feb 14, 2026

Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase

Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase

Published on: September 22, 2012

Intracerebral Hemorrhage in Mice.

Damon Klebe¹, Loretta Iniaghe¹, Sherrefa Burchell¹

¹Department of Physiology & Pharmacology, Loma Linda University, Loma Linda, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|February 23, 2018

Summary

Intracerebral hemorrhage (ICH) is a severe stroke. This study details two mouse models, autologous blood injection and collagenase infusion, to better understand ICH and test new treatments.

Keywords:

Animal model Autologous blood Collagenase Intracerebral hemorrhage Mouse Stroke

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Published on: September 22, 2012

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Published on: August 24, 2011

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Published on: October 15, 2021

Area of Science:

Neuroscience
Stroke Research
Animal Models

Background:

Intracerebral hemorrhage (ICH) is a severe stroke subtype with high mortality and morbidity.
Currently, no effective treatments exist to improve survival or quality of life for ICH survivors.
Developing robust models is crucial for understanding ICH pathophysiology and evaluating therapies.

Purpose of the Study:

To describe two distinct mouse models for inducing intracerebral hemorrhage.
To provide tools for investigating ICH mechanisms and potential treatments.

Main Methods:

Autologous blood double-injection model: Mimics spontaneous ICH by injecting whole blood into the brain parenchyma.
Collagenase infusion model: Uses enzymatic degradation to create a focal brain hemorrhage.

Main Results:

Both models successfully induce intracerebral hemorrhage in mice.
These models allow for the study of acute and chronic effects of ICH.
Detailed procedural descriptions are provided for reproducibility.

Conclusions:

The described autologous blood and collagenase infusion models offer valuable platforms for ICH research.
These models can aid in the discovery and testing of novel therapeutic strategies for intracerebral hemorrhage.