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Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase
Published on: September 22, 2012
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Intracerebral Hemorrhage in Mice
Damon Klebe1, Loretta Iniaghe1, Sherrefa Burchell1
1Department of Physiology & Pharmacology, Loma Linda University, Loma Linda, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|February 23, 2018
Summary
Intracerebral hemorrhage (ICH) is a severe stroke. This study details two mouse models, autologous blood injection and collagenase infusion, to better understand ICH and test new treatments.
Area of Science:
- Neuroscience
- Stroke Research
- Animal Models
Background:
- Intracerebral hemorrhage (ICH) is a severe stroke subtype with high mortality and morbidity.
- Currently, no effective treatments exist to improve survival or quality of life for ICH survivors.
- Developing robust models is crucial for understanding ICH pathophysiology and evaluating therapies.
Purpose of the Study:
- To describe two distinct mouse models for inducing intracerebral hemorrhage.
- To provide tools for investigating ICH mechanisms and potential treatments.
Main Methods:
- Autologous blood double-injection model: Mimics spontaneous ICH by injecting whole blood into the brain parenchyma.
- Collagenase infusion model: Uses enzymatic degradation to create a focal brain hemorrhage.
Main Results:
- Both models successfully induce intracerebral hemorrhage in mice.
- These models allow for the study of acute and chronic effects of ICH.
- Detailed procedural descriptions are provided for reproducibility.
Conclusions:
- The described autologous blood and collagenase infusion models offer valuable platforms for ICH research.
- These models can aid in the discovery and testing of novel therapeutic strategies for intracerebral hemorrhage.
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