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A Murine Model of Myocardial Ischemia-Reperfusion Injury

Zhaobin Xu1, Kevin E McElhanon1, Eric X Beck1

  • 1Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute, Ohio State University Wexner Medical Center, Columbus, OH, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 23, 2018
PubMed

Ligation of the left anterior descending (LAD) coronary artery in the mouse heart is a widely used model to simulate myocardial infarction and ischemia-reperfusion injury. Here we describe a ligation technique routinely performed in our laboratory to induce myocardial infarction that may be used to study ischemia-reperfusion injury in the myocardium. The methods described enhance location of the LAD coronary artery to allow for accurate ligation, thus increasing reproducibility of infarct size and location.

Keywords:
LAD ligationLeft anterior descending coronary arteryMyocardial infarctionMyocardial ischemia–reperfusionPermanent ligation

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