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Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker
Published on: March 31, 2018
The effect of hydroxyapatite nanoparticles on adipogenic differentiation of human mesenchymal stem cells
Xing Yang1, Yuanyuan Li2, Qianli Huang1
1State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, 100084, China.
Abstract:
Due to its excellent biocompatibility, nanosized hydroxyapatite (nHA) has drawn much attention for various applications in biomedical fields. There are growing concerns about its biosecurity; however, little is known about its effects on adipogenesis. In the present study, nHA with three different sizes were synthesized, and the in vitro effects of nHA on cell proliferation and adipogenic differentiation of human mesenchymal stem cells (hMSCs) were investigated. The results clearly show that nHA does not affect the cell viability, the lipids droplets formation, triglyceride (TG) synthesis, and the expression of adipogenic marker genes/proteins of hMSCs at concentrations lower than 50 μg/mL. It is concluded that the adipogenic differentiation potential of hMSCs is not affected by nHA at noncytotoxic concentrations. These will provide a reference for the applications of nHA in biomedical fields. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1822-1831, 2018.
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