Development and characterization of HBsAg-loaded Eudragit nanoparticles for effective colonic immunization

Area of Science:

• Pharmaceutical Sciences
• Vaccinology
• Nanotechnology

Background:

• Oral vaccine delivery faces challenges in reaching the colonic mucosa for effective immune stimulation.
• Developing stable, targeted nanoparticles is crucial for enhancing oral vaccine efficacy.

Purpose of the Study:

• To optimize Eudragit nanoparticles for colon targeting of oral vaccines.
• To evaluate the immune response generated by colon-targeted nanoparticles.

Main Methods:

• Response surface methodology (RSM) with central composite design (CCD) for formulation optimization.
• Characterization of nanoparticles including particle size, entrapment efficiency, and PDI.
• In-vitro release studies and animal imaging to confirm colon targeting and immune response.

Main Results:

• Optimized Eudragit nanoparticles (730.4 nm, 58.38% entrapment, 0.185 PDI) were successfully formulated.
• Antigen integrity was maintained throughout the formulation process.
• In-vivo imaging confirmed effective colon distribution and enhanced immune response.

Conclusions:

• Eudragit nanoparticles demonstrate significant potential for colon targeting of oral vaccines.
• This approach can improve prophylactic humoral and mucosal immunity via oral immunization.