Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Study Designs in Epidemiology01:20

Study Designs in Epidemiology

1.0K
Epidemiological study designs are fundamental tools for investigating the distribution, determinants, and control of health conditions in populations. They help researchers understand the relationships between exposures and outcomes, and they broadly fall into two categories: "observational" and "experimental" studies.
Observational studies are those where the researcher does not intervene but rather observes natural variations. They include cross-sectional, cohort, and...
1.0K
Study Design in Statistics01:15

Study Design in Statistics

10.1K
A study design is a set of techniques that allow a researcher to collect and analyze data from different variables defined for a specific research problem. Statistics is commonly for effective study design and more robust experiments,
Does aspirin reduce the risk of heart attacks? Is one brand of fertilizer more effective at growing roses than another? Is fatigue as dangerous to a driver as the influence of alcohol? Questions like these are answered using randomized experiments with proper...
10.1K
Factorial Design02:01

Factorial Design

14.2K
Factorial Analysis is an experimental design that applies Analysis of Variance (ANOVA) statistical procedures to examine a change in a dependent variable due to more than one independent variable, also known as factors. Changes in worker productivity can be reasoned, for example, to be influenced by salary and other conditions, such as skill level. One way to test this hypothesis is by categorizing salary into three levels (low, moderate, and high) and skills sets into two levels (entry level...
14.2K
Group Design02:01

Group Design

10.8K
The most basic experimental design involves two groups: the experimental group and the control group. The two groups are designed to be the same except for one difference— experimental manipulation. The experimental group gets the experimental manipulation—that is, the treatment or variable being tested—and the control group does not. Since experimental manipulation is the only difference between the experimental and control groups, we can be sure that any differences between...
10.8K
Nursing Implementation01:15

Nursing Implementation

6.3K
Implementation is the execution of the nursing care plan developed during the planning phase.
The five steps to implementing effective nursing care include reassessing the patient, reviewing and revising the existing nursing care plan, organizing the resources and care delivery, anticipating and preventing complications, and implementing nursing interventions.
6.3K
Bioavailability Study Design: Single Versus Multiple Dose Studies01:11

Bioavailability Study Design: Single Versus Multiple Dose Studies

253
Bioavailability studies are essential for understanding how a drug is absorbed, distributed, metabolized, and excreted in the body. These studies assess the extent and rate at which the active pharmaceutical agent becomes available at the site of action. The design of bioavailability studies can involve single-dose or multiple-dose regimens, each with distinct advantages and limitations.Single-dose studies are the preferred approach due to their simplicity and reduced drug exposure for...
253

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stunting, underweight and thinness in internationally adopted children: prevalence and associated factors in a large cohort study.

European journal of pediatrics·2026
Same author

Multisite study: Predicting Lewy body disease using skin biopsy α-synuclein seed amplification assays.

Journal of neuropathology and experimental neurology·2026
Same author

Brain-Only Versus GI-Only Synucleinopathy: A Comprehensive Autopsy Study With Both IHC and SAA.

medRxiv : the preprint server for health sciences·2026
Same author

Accurate Genomic Predictions for Chronic Wasting Disease in North American Elk.

G3 (Bethesda, Md.)·2026
Same author

Differential patterns of central synucleinopathy and catecholaminergic abnormalities in Lewy body diseases and multiple system atrophy.

Parkinsonism & related disorders·2026
Same author

Correction to "Design of Tau Aggregation Inhibitors Using Iterative Machine Learning and a Polymorph-Specific Brain-Seeded Fibril Amplification Assay".

Journal of the American Chemical Society·2026

Related Experiment Video

Updated: Feb 14, 2026

Protein Misfolding Cyclic Amplification of Prions
10:12

Protein Misfolding Cyclic Amplification of Prions

Published on: November 7, 2012

20.2K

Design, implementation, and interpretation of amplification studies for prion detection.

Nicholas J Haley1, Jürgen A Richt2, Kristen A Davenport3

  • 1a Department of Microbiology and Immunology , Midwestern University , Glendale , AZ , USA.

Prion
|February 23, 2018
PubMed
Summary

Developing structured frameworks for protein misfolding cyclic amplification (PMCA) and real time quaking-induced conversion (RT-QuIC) assays improves prion disease diagnostics. This approach enhances the transition from pilot studies to clinical trials for human and animal prion diseases.

Keywords:
Creutzfeldt-Jakob diseasePMCART-QuICamplificationchronic wasting diseaseprionscrapie

More Related Videos

Detection of Abnormal Prion Protein by Immunohistochemistry
06:38

Detection of Abnormal Prion Protein by Immunohistochemistry

Published on: May 5, 2023

3.8K
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
09:50

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Published on: September 29, 2017

14.4K

Related Experiment Videos

Last Updated: Feb 14, 2026

Protein Misfolding Cyclic Amplification of Prions
10:12

Protein Misfolding Cyclic Amplification of Prions

Published on: November 7, 2012

20.2K
Detection of Abnormal Prion Protein by Immunohistochemistry
06:38

Detection of Abnormal Prion Protein by Immunohistochemistry

Published on: May 5, 2023

3.8K
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
09:50

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Published on: September 29, 2017

14.4K

Area of Science:

  • Neuroscience
  • Biochemistry
  • Medical Diagnostics

Background:

  • Transmissible spongiform encephalopathies (TSEs) require accurate diagnostic methods.
  • Protein misfolding cyclic amplification (PMCA) and real time quaking-induced conversion (RT-QuIC) show promise for prion disease detection.
  • Limited published guidance exists for the structured development and interpretation of these amplification assays.

Purpose of the Study:

  • To provide a framework for the structured development, implementation, and interpretation of PMCA and RT-QuIC assays.
  • To facilitate the efficient progression of pilot studies into diagnostic trials for prion diseases.
  • To establish a foundation for applying these methods to other protein misfolding disorders.

Main Methods:

  • The proposed framework integrates principles from diagnostic medicine.
  • Key components include defining analytical and diagnostic sensitivity and specificity.
  • Emphasis is placed on a priori amplification strategy development and robust experimental design.

Main Results:

  • The manuscript outlines a structured approach to prion amplification assay development.
  • This framework aims to standardize experimental design and interpretation.
  • The goal is to enhance the reliability and applicability of PMCA and RT-QuIC.

Conclusions:

  • A structured framework is essential for advancing prion amplification assays from research to diagnostic applications.
  • This approach will improve the diagnosis of human and animal prion diseases.
  • The framework may also guide the development of diagnostic tools for other neurodegenerative protein misfolding diseases like Alzheimer's and Parkinson's.