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Updated: Feb 14, 2026
Transient Transduction of the Strobilated Forms of Echinococcus granulosus
Published on: September 16, 2022
[Cloning, expression and serological evaluation of H3 protein from Echinococcus granulosus]
Zhu Hui-Hui1, Gao Chun-Hua1, Wang Jun-Yun1
1National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory on Parasite and Vector Biology, Ministry of Health, National Center for International Research on Tropical Disease, Ministry of Science and Technology, WHO Collaborating Center for Tropical Diseases, Shanghai 200025, China.
Objective:
To clone and express the basement membrane specific heparan sulfate proteoglycan core protein (H3), and to evaluate its effect in detection of human cystic echinococcosis (CE).
Methods:
The H3 gene immunoscreened from the cDNA library was cloned into pGEX-4T vector. The recombinant plasmid pGEX-3X-AgB8/3 was transformed into Escherichia coli BL21 strains and induced by isopropyl-β-D-thiogalactopyranoside (IPTG). Then the expressed recombinant protein was purified by affinity chromatography and its effect in the detection of CE was evaluated by ELISA. Meanwhile, the effects of H3 and two antigens that the research group prepared before (purified HCF and rAgB8/2) in CE detection were compared.
Results:
The plasmid pGEX-4T-H3 was successfully constructed and H3 was successfully expressed in prokaryotic cells. The sensitivities of the recombinant H3, purified HCF and rAgB8/2 in CE detection were 84.0% (68/81), 90.1% (73/81) and 77.8% (63/81) respectively, and there was no statistical difference among them (χ2 = 4.58, P > 0.05). The cross reactions of recombinant H3 with the sera of the patients with CE, cysticercosis and schistosomiasis were 63.3% (19/30), 16.7% (5/30) and 5.0% (1/20) respectively, and the cross reaction was 0 with the sera of healthy people. The specificities of recombinant H3, purified HCF, and rAgB8/2 were 80.8% (105/130), 71.5% (93/130) and 82.3% (107/130) respectively, and there were no statistical difference among them (χ2 = 5.71, P > 0.05).
Conclusions:
The recombinant H3 is a potential diagnostic antigen for CE detecting.
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