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Xenopus: An alternative model system for identifying muco-active agents.

Hyo Jung Sim1, Sang-Hyun Kim2, Kyung-Jae Myung1,3

  • 1School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan, South Korea.

Plos One
|February 23, 2018
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Summary

Researchers developed a novel high-throughput screening model using Xenopus laevis embryos to find new mucus-regulating drugs. This method identified narasin, which effectively reduces mucus secretion in both embryos and human lung cells.

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Area of Science:

  • Respiratory Medicine
  • Developmental Biology
  • Pharmacology

Background:

  • The airway epithelium is crucial for defense against environmental threats.
  • Respiratory diseases like COPD and asthma disrupt mucus clearance.
  • Current treatments target inflammation or mucus viscosity, not goblet cell mucus production.

Purpose of the Study:

  • To develop a high-throughput in vivo model for screening muco-active agents.
  • To identify novel reagents that directly regulate airway goblet cell mucus secretion.
  • To overcome limitations of existing screening methods for muco-regulators.

Main Methods:

  • Development of a high-throughput in vivo screening system using Xenopus laevis embryos.
  • Testing mucus secretion under various conditions to establish a screening strategy.
  • Validation of findings using the human lung epithelial cell line Calu-3.

Main Results:

  • Identification of narasin as a novel muco-regulator through the developed screening technique.
  • Narasin treatment significantly reduced mucus secretion in developing Xenopus embryos.
  • Human Calu-3 lung epithelial cells showed a similar response to narasin, confirming the model's efficacy.

Conclusions:

  • A novel high-throughput in vivo model using Xenopus laevis embryos is effective for discovering muco-active reagents.
  • Narasin emerges as a promising candidate for regulating mucus secretion.
  • This platform facilitates the discovery of new therapeutic strategies for mucus-related respiratory disorders.