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Updated: Feb 14, 2026

Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues
Published on: February 27, 2021
Arabinogalactan proteins and their sugar chains: functions in plant reproduction, research methods, and biosynthesis
Shihao Su1, Tetsuya Higashiyama2,3
1Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, Aichi, 464-8601, Japan.
Abstract:
The arabinogalactan protein (AGP) family is one of the most complex protein families and is ubiquitous in the plant kingdom. Moreover, it has been demonstrated to play various roles during plant reproduction. A typical AGP contains a hydroxyproline-rich core protein with high heterogeneity and varying numbers of polysaccharide side chains. However, the functions of the polysaccharide components (i.e. AG sugar chains) remain largely unknown due to the general difficulties associated with studying sugar chains in glycobiology. In recent years, methodological breakthroughs have resulted in substantial progress in AGP research. Here, we summarise the multiple roles of AGPs during plant gametophyte development and male-female communication, with a focus on recent advances. In addition, we discuss the analytical tools used in AGP research, and the biosynthesis and function of AG sugar chains. A comprehensive understanding of the AGP family will help clarify the mechanisms precisely controlling reproductive processes.
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