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Characterization of highly branched dextran produced by Leuconostoc citreum B-2 from pineapple fermented product

Fang Feng1, Qingqing Zhou1, Yanfang Yang1

  • 1School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.

International Journal of Biological Macromolecules
|February 23, 2018
PubMed

Insights

Researchers isolated Leuconostoc citreum B-2, producing a high yield of exopolysaccharide (EPS). This pineapple-derived EPS, composed of glucose, shows potential for food, cosmetic, and pharmaceutical applications.

Area of Science:

  • Microbiology
  • Biochemistry
  • Food Science

Background:

  • Exopolysaccharides (EPS) are microbial polymers with diverse industrial applications.
  • Pineapple fermentation is a source of novel microbial strains and bioactive compounds.

Purpose of the Study:

  • To isolate and characterize a novel exopolysaccharide (EPS) from a pineapple-derived strain of Leuconostoc citreum B-2.
  • To evaluate the physicochemical properties and potential applications of the isolated EPS.

Main Methods:

  • Isolation and cultivation of Leuconostoc citreum B-2 in Man-Rogosa-Sharpe (MRS) medium.
  • Characterization of EPS using Gas Chromatography (GC), Fourier-transform infrared (FT-IR) spectroscopy, High-Performance Size-Exclusion Chromatography (HPSEC), Nuclear Magnetic Resonance (NMR) spectroscopy, and Scanning Electron Microscopy (SEM).
  • Determination of monosaccharide composition, molecular weight, and functional properties (water solubility, water holding capacity).

Main Results:

  • A high yield of exopolysaccharide (EPS) (28.3 g/L) was obtained from Leuconostoc citreum B-2.
  • The EPS was primarily composed of glucose units with a molecular weight of 3.77×10^6 Da.
  • Structural analysis revealed a backbone of α-(1→6) linked d-glucopyranose with α-(1→3) and α-(1→2) branching; SEM showed irregular sheets with a compact structure. Water solubility index and water holding capacity were 80% and 450%, respectively.

Conclusions:

  • The characterized exopolysaccharide (EPS) from Leuconostoc citreum B-2 exhibits favorable physicochemical properties.
  • The EPS demonstrates significant potential for application in the food, cosmetic, and pharmaceutical industries due to its composition and functional characteristics.
Keywords:
DextranExopolysaccharideLeuconostoc citreumStructural characterization

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