Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Conversion02:08

Gene Conversion

10.7K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
10.7K
Gene Conversion02:08

Gene Conversion

3.1K
3.1K
Lipid Digestion01:06

Lipid Digestion

99.9K
Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.
99.9K
What are Lipids?01:38

What are Lipids?

221.2K
Overview
221.2K
Conversion of Units01:36

Conversion of Units

37.1K
Sometimes, there is a need to convert from one unit to another one. For instance, reading a cookbook in which quantities are expressed in units of liters or ounces may require conversion of quantities to cups. Or, when looking up directions on how to get to a location, we may be interested to know how many miles we are going to walk. In this case, we would have to convert units of feet or meters to miles.
The first step in the unit conversion is to list the given units and the units required...
37.1K
Structure of Lipids03:38

Structure of Lipids

99.4K
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic...
99.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Evaluation of plasma chill spray technology for the inactivation of wet and dry biofilms of Escherichia coli on stainless steel surfaces" [Food Microbiol. 139 (2026) 105122].

Food microbiology·2026
Same author

Medicinal Insights Into FLT3 Inhibitors as Anticancer Agents: Current Status and Future Direction.

Archiv der Pharmazie·2026
Same author

Predicting band gap from chemical composition: a comparative study of machine learning and deep learning models.

Scientific reports·2026
Same author

Cluster-size-dependent cooperativity in sulfur-centred hydrogen bonds: ethane-1,2-dithiol with HF, H<sub>2</sub>O, and NH<sub>3</sub>.

Physical chemistry chemical physics : PCCP·2026
Same author

Comment on "Using mobile applications for body composition analysis: A technical review of an artificial intelligence-based tool".

Clinical nutrition ESPEN·2026
Same author

Evaluation of plasma chill spray technology for the inactivation of wet and dry biofilms of Escherichia coli on stainless steel surfaces.

Food microbiology·2026

Related Experiment Video

Updated: Feb 14, 2026

Author Spotlight: Employing Green-Chemistry Principles for Safe and Sustainable Synthesis of Biodiesels
04:40

Author Spotlight: Employing Green-Chemistry Principles for Safe and Sustainable Synthesis of Biodiesels

Published on: April 19, 2024

1.6K

Recent developments of downstream processing for microbial lipids and conversion to biodiesel.

Sravan Kumar Yellapu1, Bharti1, Rajwinder Kaur1

  • 1INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada.

Bioresource Technology
|February 24, 2018
PubMed
Summary

Developing efficient biodiesel production requires innovative lipid extraction from wet microbial biomass. This review explores cell disruption and purification methods for sustainable biofuel solutions, addressing the food vs. fuel crisis.

Keywords:
Biodiesel purificationBiomass harvestingCell wall disruptionLipid recoveryTransesterificationWet biomass

More Related Videos

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
09:10

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Published on: June 24, 2016

21.4K
Assembly and Tracking of Microbial Community Development within a Microwell Array Platform
09:24

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

Published on: June 6, 2017

9.7K

Related Experiment Videos

Last Updated: Feb 14, 2026

Author Spotlight: Employing Green-Chemistry Principles for Safe and Sustainable Synthesis of Biodiesels
04:40

Author Spotlight: Employing Green-Chemistry Principles for Safe and Sustainable Synthesis of Biodiesels

Published on: April 19, 2024

1.6K
Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
09:10

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Published on: June 24, 2016

21.4K
Assembly and Tracking of Microbial Community Development within a Microwell Array Platform
09:24

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

Published on: June 6, 2017

9.7K

Area of Science:

  • Biotechnology
  • Renewable Energy
  • Biorefining

Background:

  • Growing global population and resource depletion necessitate innovative solutions for basic needs.
  • Biodiesel is a sustainable biofuel addressing the
  • food vs. fuel crisis
  • and is derived from microbial lipids.
  • Lipid extraction from oleaginous microbes is crucial for biodiesel production within biorefineries.

Purpose of the Study:

  • To critically review cell disruption, lipid recovery, and purification methods for extracting lipids from wet microbial biomass.
  • To support the development of efficient and scalable industrial processes for biodiesel production.
  • To highlight the advantages of direct wet cell-biomass lipid extraction over dry methods, avoiding costly dehydration steps.

Main Methods:

  • Literature review focusing on cell disruption techniques for microbial biomass.
  • Analysis of lipid recovery and purification strategies.
  • Evaluation of methods for direct lipid extraction from wet cell biomass.

Main Results:

  • Direct lipid extraction from wet cell biomass is more economical than using dry biomass, as it bypasses expensive dehydration processes.
  • Current wet cell biomass lipid extraction methods require further research and development for industrial scalability.
  • Efficient cell disruption and purification are key to successful lipid recovery.

Conclusions:

  • Establishing robust, industrially applicable methods for wet cell biomass lipid extraction is essential for commercializing biodiesel production.
  • Further research is needed to optimize cell disruption, lipid recovery, and purification for efficient transesterification.
  • Sustainable biofuel production via biorefining hinges on advancements in microbial lipid extraction technologies.