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Updated: Feb 14, 2026

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
Published on: March 15, 2019
Next-Generation Sequencing of Antibody Display Repertoires
Romain Rouet1, Katherine J L Jackson1, David B Langley1
1Garvan Institute of Medical Research, Sydney, NSW, Australia.
Abstract:
In vitro selection technology has transformed the development of therapeutic monoclonal antibodies. Using methods such as phage, ribosome, and yeast display, high affinity binders can be selected from diverse repertoires. Here, we review strategies for the next-generation sequencing (NGS) of phage- and other antibody-display libraries, as well as NGS platforms and analysis tools. Moreover, we discuss recent examples relating to the use of NGS to assess library diversity, clonal enrichment, and affinity maturation.
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