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Updated: Feb 14, 2026
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Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations
Published on: June 23, 2022
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Rapid Analysis of Cell-Nanoparticle Interactions using Single-Cell Raman Trapping Microscopy.
Maria Steinke1, Florian Zunhammer2, Elisavet I Chatzopoulou3
1Fraunhofer Institute for Silicate Research ISC, c/o University Hospital Würzburg, Chair of Tissue Engineering and Regenerative Medicine, Röntgenring 11, 97070, Würzburg, Germany.
Angewandte Chemie (International Ed. in English)
|February 24, 2018
Summary
Researchers developed a rapid Raman trapping microscopy method to efficiently assess iron oxide nanoparticle safety for stem cell labeling. This technique quickly distinguishes safe from cytotoxic particles, crucial for clinical applications.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Cell Biology
Background:
- Iron oxide nanoparticles are explored for stem cell labeling in preclinical research.
- Novel nanoparticle candidates are needed for safe clinical translation.
- Current safety and interaction analyses are time-consuming.
Purpose of the Study:
- To develop an efficient method for analyzing cell-particle interactions and cytotoxicity.
- To guide the development of safe iron oxide nanoparticles for clinical use.
- To enable rapid discrimination between non-hazardous and cytotoxic nanoparticles.
Main Methods:
- Combined Raman trapping microscopy with fluidic chips for single-cell analysis.
- Labeling of stem cells with different aminated iron oxide particles.
- Multivariate data analysis of Raman spectra to identify cell-particle interactions and cytotoxicity.
Main Results:
- Raman signal differences were clearly assigned to cell-particle interactions and cytotoxicity.
- The approach achieved over 95% correct classification of spectra.
- Demonstrated rapid discrimination of nanoparticle safety.
Conclusions:
- The developed Raman trapping microscopy approach is efficient for evaluating nanoparticle safety.
- This method is a prerequisite for the clinical application of iron oxide nanoparticles.
- Enables faster development and selection of safe nanoparticles for stem cell labeling.
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