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Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm
Published on: November 19, 2020
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Proteogenomic Analysis to Identify Missing Proteins from Haploid Cell Lines.
Seung-Eun Lee1,2, JongKeon Song1, Korbinian Bösl3
1Department of Applied Chemistry College of Applied Science, Kyung Hee University, Yongin-si, Republic of Korea.
Proteomics
|February 24, 2018
Summary
Researchers hunted for missing human proteins using expression proteomics in HAP1 and KBM-7 cells. This approach identified nine previously missing proteins and six novel protein-coding regions, advancing proteome characterization.
Area of Science:
- Proteomics and Genomics
- Human Proteome Project
- Mass Spectrometry
Background:
- The Chromosome-centric Human Proteome Project aims to identify all human proteins.
- Over 2,600 proteins remain 'missing' (unidentified by mass spectrometry).
- Missing proteins may be low abundance or tissue-specific (e.g., testis).
Purpose of the Study:
- To identify missing proteins using expression proteomics.
- To explore novel protein-coding regions using proteogenomic analysis.
Main Methods:
- Expression proteomics on near-haploid HAP1 and KBM-7 cell lines.
- Analysis of 200 mass spectrometry files using LTQ Orbitrap Velos.
- Proteogenomic analysis of unmatched spectra against RNA-Seq derived databases.
Main Results:
- Identification of over 10,000 proteins from HAP1 and KBM-7 cells.
- Discovery of nine previously missing human proteins.
- Identification of six novel protein-coding regions through proteogenomic analysis.
Conclusions:
- Expression proteomics is effective for identifying missing proteins.
- Proteogenomic analysis can uncover unannotated protein-coding regions.
- This study contributes to the comprehensive characterization of the human proteome.
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