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Isolation and molecular characterization of Corynebacterium pseudotuberculosis from sheep and goats in Mexico
Jorge Antonio Varela Guerrero1, Roberto Montes de Oca Jiménez1, Jorge Acosta Dibarrat1
1Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Km 15.5 Carretera Toluca, Atlacomulco, Estado de México, C.P. 50200, Mexico.
Abstract:
The pathogenic bacteria of Corynebacterium pseudotuberculosis caused a chronic contagious infectious disease of the caseous lymphadenitis or pseudotuberculosis. Globally, isolates obtained from different injuries that affect sheep and goats, have been identified by fully or partially gene sequencing. However, in Mexico there is no complete study to identify by molecular and phylogenetic techniques the circulating isolates as well as its virulence factors. Therefore, in the present study we reported the identification of 57 isolates of C. pseudotuberculosis by bacteriological tests and the amplification of 16S rRNA, rpoB and pld genes, as well as, genes involved in virulence and pathogenicity: Fag A, Fag B, Fag C, Fag D and hsp60. Phylogenetic analysis was performed based on the partial sequence of the rpoB gene. Genes involved in virulence and pathogenicity were identified in the 98.2% of the isolates. Regarding the phylogenetic analysis, were identified the species and subspecies to which they belong of all the tested isolates. The phenotypic and genotypic characterization will allow to establish preventive and prophylactic measures aimed to the creation of effective immunogens against Corynebacterium pseudotuberculosis.
Insights
This study identifies Corynebacterium pseudotuberculosis bacterial isolates from Mexican livestock using molecular methods. Findings reveal key virulence factors and genetic relationships, aiding disease prevention strategies.
Area of Science:
- Veterinary Microbiology
- Bacterial Pathogenesis
- Molecular Epidemiology
Background:
- Corynebacterium pseudotuberculosis causes caseous lymphadenitis in sheep and goats globally.
- Molecular identification of C. pseudotuberculosis isolates is lacking in Mexico.
- Understanding virulence factors is crucial for disease control.
Purpose of the Study:
- To molecularly identify and phylogenetically characterize C. pseudotuberculosis isolates from Mexico.
- To detect genes associated with virulence and pathogenicity in these isolates.
- To inform the development of preventive and prophylactic measures.
Main Methods:
- Bacteriological tests for isolate identification.
- Gene amplification of 16S rRNA, rpoB, pld, Fag A-D, and hsp60.
- Phylogenetic analysis using partial rpoB gene sequences.
Main Results:
- Identified 57 C. pseudotuberculosis isolates.
- Detected virulence and pathogenicity genes in 98.2% of isolates.
- Determined the species and subspecies of all isolates through phylogenetic analysis.
Conclusions:
- Phenotypic and genotypic characterization provides a foundation for C. pseudotuberculosis control in Mexico.
- Identification of virulence factors is essential for developing targeted immunogens.
- Molecular and phylogenetic data are vital for understanding bacterial epidemiology and disease management.
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