Linking nitrous oxide emissions to population dynamics of nitrifying and denitrifying prokaryotes in four full-scale...

Ammonia-oxidizing bacteria (AOB), ammonia oxidizing archaea (AOA) and N₂O-reducing denitrifiers were measured by quantitative real-time PCR (qPCR) in activated sludge samples from four full-scale wastewater treatment plants (WWTPs) in South Spain, and their abundances were linked to the generation of N₂O in the samples using multivariate analysis (Non-metric multidimensional scaling, MDS, and BIO-ENV). The average abundances of AOA remained in similar orders of magnitude in all WWTPs (10⁶ copies amoA/L activated sludge mixed liquor), while significant differences were detected for AOB (10⁵-10⁹copies amoA/L) and N₂O-reducers (10⁷-10¹⁰copies nosZ/L). Average N₂O emissions measured in activated sludge samples ranged from 0.10 ± 0.05 to 6.49 ± 8.89 mg N₂O-N/h/L activated sludge, and were strongly correlated with increased abundances of AOB and lower counts of N₂O-reducers. A significant contribution of AOA to N₂O generation was unlikely, since their abundance correlated negatively to N₂O emissions. AOB abundance was favoured by higher NO₃^- and NO₂^-concentrations in the activated sludge.