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Related Experiment Video

Updated: Feb 10, 2026

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
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Coherent two-dimensional fluorescence micro-spectroscopy.

Sebastian Goetz, Donghai Li, Verena Kolb

    Optics Express
    |February 25, 2018
    PubMed
    Summary
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    We developed a new coherent two-dimensional (2D) fluorescence micro-spectroscopy technique. This method uses laser pulses to analyze microscopic variations in heterogeneous systems with high spatial resolution.

    Area of Science:

    • Optics and Photonics
    • Materials Science
    • Spectroscopy

    Background:

    • Investigating microscopic variations in heterogeneous systems is crucial for understanding material properties.
    • Nonlinear optical responses at surfaces provide detailed information about material characteristics.
    • Sub-micron spatial resolution is often required to probe localized phenomena.

    Purpose of the Study:

    • To develop and demonstrate a novel coherent two-dimensional (2D) fluorescence micro-spectroscopy technique.
    • To achieve sub-micron spatial resolution for probing nonlinear optical responses at surfaces.
    • To analyze microscopic variations within heterogeneous materials.

    Main Methods:

    • Utilized an LCD-based pulse shaper in 4f geometry to generate collinear laser pulse trains.

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  • Employed 12-fs visible/NIR laser pulses focused by an NA = 1.4 immersion-oil microscope objective.
  • Applied phase cycling analysis to 2D fluorescence spectra.

    • Main Results:

    • Successfully developed coherent 2D fluorescence micro-spectroscopy with sub-micron spatial resolution.
    • Demonstrated the technique's capability to probe nonlinear optical responses via fluorescence detection.
    • Presented 2D spectra analyzed as a function of position on a nanostructured F16ZnPc thin film.

    Conclusions:

    • The developed coherent 2D fluorescence micro-spectroscopy is effective for investigating microscopic variations.
    • The technique offers high spatial resolution for surface analysis.
    • This method provides a powerful tool for characterizing heterogeneous materials at the microscale.