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Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
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Preparation of PEGylated cationic nanoliposome-siRNA complexes for cancer therapy

Fateme Haghiralsadat1, Ghasem Amoabediny2,3, Samira Naderinezhad2

  • 1a Department of Life Science Engineering , Faculty of New Sciences and Technologies, University of Tehran , Tehran , Iran.

Artificial Cells, Nanomedicine, and Biotechnology
|February 25, 2018
PubMed

Insights

Stealth PEGylated liposomes enhance small interfering RNA (siRNA) delivery and stability. These novel liposome formulations show great potential for systemic siRNA delivery and improved in vivo stability.

Area of Science:

  • Biotechnology
  • Nanomedicine
  • Gene Delivery

Background:

  • Viral vectors pose challenges for gene delivery.
  • Liposome instability hinders effective small interfering RNA (siRNA) delivery.
  • Non-viral vectors like cationic liposomes are explored to overcome these limitations.

Purpose of the Study:

  • To develop stealth PEGylated liposome formulations for enhanced siRNA delivery.
  • To investigate the impact of PEGylation on liposome characteristics and siRNA complex stability.
  • To evaluate the efficacy of PEGylated liposomes in gene silencing applications.

Main Methods:

  • Formulation of stealth PEGylated liposomes.
  • Characterization of liposome size, zeta potential, and polydispersity index.
  • Assessment of siRNA loading efficiency and long-term stability of siRNA-liposome complexes.
  • Evaluation of intracellular siRNA delivery and gene silencing in MG-63 osteosarcoma cells.

Main Results:

  • PEGylated liposomes demonstrated efficient siRNA loading and prevented aggregation.
  • siRNA-liposome complexes exhibited excellent long-term stability at 4°C for at least 6 months with minimal siRNA release (1-5%).
  • PEGylation significantly improved liposome parameters and enhanced intracellular siRNA delivery, leading to successful J1P1 gene silencing in MG-63 cells.

Conclusions:

  • Novel PEGylated liposomes offer a promising non-viral vector for systemic siRNA delivery.
  • These formulations enhance the in vivo stability of both free siRNA and siRNA lipoplexes.
  • PEGylated liposomes represent a significant advancement in overcoming challenges in siRNA-based therapeutics.
Keywords:
MAPK8IP1/JIP1 silencingPEGylationintracellular deliverysiRNA lipoplexstability

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