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Updated: Feb 14, 2026

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography
Published on: October 17, 2018
Fused micro-computed tomography (μCT) and histological images of bone specimens
C Mourad1, K Laperre2, M Halut1
1Radiology department, Cliniques Universitaires Saint-Luc, IREC, UCL, Avenue Hippocrate 10, 1200 Woluwe-Saint-Lambert, Brussels, Belgium.
Objectives:
To describe a stepwise process to obtain fused images from micro-computed tomography (μCT) and histological images of bone specimens.
Material And Methods:
Four surgically resected human femoral heads from four patients who had total hip replacement were imaged at a spatial resolution of 12-microns by using μCT. Histological sections of four focal bone lesions including bone cyst in osteoarthritis (n=2) and subchondral bone plate fracture in osteonecrosis (n=2) were prepared and digitized. μCT images were reformatted and adjusted to match the histological images using a landmark-based visual co-registration. Fused μCT and histological images were displayed in a cine-loop video mode with a gradual transition from one image to the other.
Results:
μCT images of the four focal bone lesions could be successfully fused with the corresponding histological images with a near perfect match of the bone trabeculae.
Conclusion:
We present a stepwise process to obtain fused images from histological and reformatted μCT images of human femoral heads.
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