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Anti-differentiation non-coding RNA, ANCR, is differentially expressed in different types of brain tumors

Mahshid Malakootian1,2, Fatemeh Mirzadeh Azad1, Youssef Fouani1

  • 1Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Journal of Neuro-Oncology
|February 25, 2018
PubMed

Insights

The Anti-differentiation ncRNA (ANCR) is upregulated in malignant brain tumors and suppressed during neural differentiation. ANCR manipulation affects glioma cell apoptosis and cell cycle, suggesting its role in brain cancer development.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Neuroscience

Background:

  • Long non-coding RNAs (lncRNAs) regulate crucial cellular processes, including cancer pathways.
  • The Anti-differentiation ncRNA (ANCR) is vital for keratinocyte differentiation and maintaining epidermal progenitor cells.

Purpose of the Study:

  • To investigate the expression pattern of ANCR during neural differentiation.
  • To evaluate ANCR expression alterations in various cell types and brain tumors using RNAseq data and clinical samples.
  • To clarify ANCR's functional role in tumorigenesis by manipulating its expression in glioma cell lines.

Main Methods:

  • Quantitative reverse transcription PCR (qRT-PCR) for ANCR expression analysis.
  • Analysis of published RNAseq data and clinical brain tumor samples.
  • ANCR expression manipulation in glioma cell lines.

Main Results:

  • ANCR was significantly upregulated in more malignant and less differentiated brain tumors (P=0.03).
  • ANCR expression decreased during neural differentiation.
  • Suppression of ANCR in glioblastoma cells led to increased apoptosis and G1 cell cycle arrest.

Conclusions:

  • ANCR plays a role in glioma genesis.
  • ANCR may serve as a potential diagnostic and therapeutic target for brain cancers.
Keywords:
ANCRBrain tumorsLncRNAsTumor marker

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