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Related Concept Videos

Detailed Structure and Function of Lymph Nodes01:23

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Lymph nodes are bean-shaped structures that cluster along the lymphatic vessels in the inguinal, axillary, and cervical regions. Each node is divided into compartments by a capsule that extends trabeculae inward.
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Consider an angioplasty system featuring a catheter equipped with a turbine, a critical tool for removing plaque deposits from coronary arteries. This intricate medical device operates using a circuit model reminiscent of a dual-node RLC circuit powered by a current-controlled voltage source.
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Blood and lymph are fluid connective tissues. They contain cells, also known as formed elements, circulating in a liquid extracellular matrix, the plasma. The formed elements are derived from hematopoietic stem cells in the bone marrow. Blood and lymph connect all vital parts and carry nutrients, oxygen, and other essential molecules like antibodies.
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Related Experiment Video

Updated: Feb 14, 2026

High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes
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Imaging the Lymph Node Stroma.

Clément Ghigo1, Rebecca Gentek1, Marc Bajénoff2

  • 1Aix-Marseille University, Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'Immunologie de Marseille-Luminy (CIML), Marseille, France.

Methods in Molecular Biology (Clifton, N.J.)
|February 25, 2018
PubMed
Summary

Lymph node stromal cells organize the immune system within complex 3D networks. New high-resolution confocal imaging methods allow detailed in situ analysis of these crucial cells and their functions.

Keywords:
3D reconstructionConfocal imagingCryostat sectioningLymph nodeStromaVibratome sectioning

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Related Experiment Videos

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Intravital Imaging of the Mouse Popliteal Lymph Node
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Area of Science:

  • Immunology
  • Cell Biology
  • Microscopy

Background:

  • Lymph node (LN) stromal cells are increasingly recognized for their critical roles in immune system organization.
  • Understanding the in situ functions of LN stromal cells requires studying their complex 3D network architecture.

Purpose of the Study:

  • To describe novel, high-resolution imaging techniques for analyzing lymph node stromal cells.
  • To enable a deeper understanding of the in situ functions of lymph node stromal cells.

Main Methods:

  • Development of two distinct, complementary procedures for sample preparation and high-resolution imaging.
  • Utilizing confocal microscopy to visualize the intricate 3D networks of LN stromal cells.

Main Results:

  • Detailed visualization of lymph node stromal cell architecture at high resolution.
  • Establishment of reliable protocols for in situ analysis of these cells.

Conclusions:

  • The described methods provide powerful tools for studying lymph node stromal cell biology.
  • High-resolution in situ imaging is essential for fully elucidating the functions of LN stromal cells in immunity.