Analysis of Endogenous Peptide Pools of Physcomitrella patens Moss

Here, we report our approach to peptidomic analysis of the plant object which led to structure elucidation of the title peptides. P. patens samples were extracted under conditions preventing proteolytic digestion by endogenous proteases. The extracts were fractionated on size exclusion columns and the peptide fractions subjected to LC-MS/MS analysis. Mass spectra datasets were analyzed for the presence of peptides derived from the proteins encoded by the moss genome. Experimental details are given for each step, selected chromatograms and mass-spectra are presented in figures.