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Expression of hMLH1 and hMSH2 proteins in ameloblastomas and tooth germs

R Bologna-Molina1, V Pereira-Prado, C Sánchez-Romero

  • 1School of Dentistry, Molecular Pathology Area, Universidad de la República, Las Heras 1925, Montevideo, Uruguay, ronellbologna@odon.edu.uy.

Medicina Oral, Patologia Oral Y Cirugia Bucal
|February 25, 2018
PubMed
Abstract

Insights

Mismatch repair proteins (MMRPs) and Ki-67 expression differ between ameloblastomas and tooth germs. Altered MMRP mechanisms may influence ameloblastoma development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Dental Pathology

Background:

  • Mismatch repair proteins (MMRPs) are crucial for DNA replication error repair in proliferating cells.
  • hMSH2 and hMLH1 are key MMRPs, with diminished expression linked to DNA replication errors.
  • Ki-67 is the gold standard for assessing cell proliferation via immunohistochemistry.

Purpose of the Study:

  • To investigate the immunohistochemical expression of hMLH1, hMSH2, and Ki-67 in ameloblastomas and tooth germs.
  • To understand the role of MMRP alterations in ameloblastoma development.

Main Methods:

  • Immunohistochemical assays were performed on 80 ameloblastomas (40 solid, 40 unicystic) and 5 tooth germs.
  • Expression levels of hMLH1, hMSH2, and Ki-67 proteins were quantified.

Main Results:

  • Unicystic ameloblastomas exhibited higher MMRP expression than solid ameloblastomas.
  • Ki-67 proliferation index was inversely proportional to MMRP expression.
  • Tooth germs showed significantly higher expression of hMLH1, hMSH2, and Ki-67 compared to ameloblastomas.

Conclusions:

  • Differential MMRP and Ki-67 immunoexpression suggests MMRP alterations contribute to ameloblastoma's biological behavior.
  • Further research into MMRP mechanisms in ameloblastomas is warranted.

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