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Updated: Feb 14, 2026
Performing In Vivo and Ex Vivo Electrical Impedance Myography in Rodents
Published on: June 8, 2022
Predicting myofiber size with electrical impedance myography: A study in immature mice
Kush Kapur1, Rebecca S Taylor2, Kristin Qi2
1Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, 02115, USA.
Introduction:
Electrical impedance can be used to estimate cellular characteristics. We sought to determine whether it could be used to approximate myofiber size using standard prediction modeling approaches.
Methods:
Forty-four C57BL/6J wild-type immature mice of varying ages underwent electrical impedance myography (EIM) with a needle electrode array placed in the gastrocnemius. Animals were then humanely killed and muscle fixed, stained, and myofiber size quantified. Two different statistical prediction models were then applied.
Results:
Impedance parameters showed major variation with increasing myofiber size. The prediction models based on EIM data alone were able to predict fiber size, with errors in the range of ±69.05-78.44 µm2 (16.19%-18.40% with respect to the average myofiber size).
Discussion:
By using well-established statistical models, EIM data alone can provide a satisfactory estimate of myofiber size. Additional study of this approach for approximating myofiber size without the requirement of removing tissue for histological analysis is warranted. Muscle Nerve, 2018.
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