Red fluorescent protein (DsRFP) optimization for Entamoeba histolytica expression
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Updated: Feb 14, 2026

Development of a Negative Selectable Marker for Entamoeba histolytica
Published on: December 12, 2010
Cindy Jazmín Delgado-Galván1, Felipe Padilla-Vaca1, Fátima Berenice Ramírez Montiel1
1Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta s/n, Guanajuato, Gto. 36050, Mexico.
Abstract:
Entamoeba histolytica genetic organization and genome structure is complex and under intense research. The genome is fully sequenced, and several tools have been developed for the molecular study of this organism. Nevertheless, good protein tracking tags that are easy to measure and image, like the fluorescent proteins are lacking. In this report, we codon-optimized the red fluorescent protein from the coral Discosoma striata (DsRFP) for its use in E. histolytica and demonstrated functionality in vivo. We envision that this protein can be widely used for the development of transcriptional reporter systems and protein-tagging applications.
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