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Advancing translational research and precision medicine with targeted proteomics
Anuli Christiana Uzozie1, Ruedi Aebersold2
1Institute of Molecular Systems Biology, ETH Zürich, 8093 Zürich, Switzerland; BC Children's Research Institute, University of British Columbia, 950 West 28th Avenue, Vancouver, BC V5Z 4H4, Canada.
Journal of Proteomics
|February 25, 2018
Summary
Quantitative mass spectrometry advances proteomics beyond expression profiling to spatial and temporal organization. Targeted proteomics offers new avenues for biomarker discovery and precision medicine development.
Area of Science:
- Proteomics
- Biomarker Discovery
- Precision Medicine
Background:
- Quantitative mass spectrometry has evolved proteomics from protein expression profiling to detailed spatial and temporal proteome organization.
- Population heterogeneity challenges precision therapy and personalized medicine, impacting biomarker performance and therapeutic efficacy.
- Cellular proteome adaptation to signals defines phenotype, influenced by genome, transcriptome, and environment.
Purpose of the Study:
- To review advances in targeted proteomics for cellular proteome quantification.
- To explore incorporating targeted proteomics data into biomarker evaluation and precision medicine.
- To address challenges hindering clinical protein biomarker success and develop precise therapeutics.
Main Methods:
- Quantitative mass spectrometry
- Targeted proteomics approaches
- Multi-layered omics data acquisition over time
Main Results:
- Shift in proteomics focus to spatial and temporal organization.
- Enabling probing of factors influencing proteome variability.
- Highlighting benefits of comprehensive, reproducible datasets for biomarker discovery.
Conclusions:
- Targeted proteomic methodologies offer untapped benefits for biomarker discovery, development, and validation.
- Implementation in translational research can overcome challenges in clinical protein biomarkers.
- Advances facilitate the development of precise therapeutics for future applications.
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