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The beneficial effects of ozone therapy in acetaminophen-induced hepatotoxicity in mice

Aysu Hayriye Tezcan1, Omur Ozturk1, Sefer Ustebay2

  • 1Kafkas University, School of Medicine, Department of Anesthesiology and Reanimation, Kars, Turkey.

Pharmacological Reports : PR
|February 25, 2018
PubMed
Abstract

Insights

Medical ozone therapy demonstrated significant therapeutic benefits for acute acetaminophen (APAP)-induced liver injury in mice. Ozone treatment reduced liver enzymes and histopathological damage, suggesting its potential as a supplementary therapy.

Area of Science:

  • Hepatology
  • Toxicology
  • Ozone Therapy

Background:

  • Acute liver injury is a significant clinical concern.
  • Acetaminophen (APAP) overdose is a leading cause of drug-induced hepatotoxicity.
  • The therapeutic potential of ozone therapy for APAP-induced liver injury requires further elucidation.

Purpose of the Study:

  • To investigate the therapeutic efficacy of medical ozone therapy in a mouse model of acute acetaminophen (APAP)-induced hepatotoxicity.
  • To evaluate the impact of ozone therapy on liver enzyme levels and histopathological changes associated with APAP-induced liver damage.

Main Methods:

  • Twenty-four mice were divided into control, APAP-induced hepatotoxicity, and APAP + ozone therapy groups.
  • Hepatotoxicity was induced by a single intraperitoneal dose of APAP (300mg/kg).
  • Ozone therapy was administered intraperitoneally twice daily to the treatment group; liver enzymes and histopathology were assessed on day four.

Main Results:

  • APAP administration significantly induced hepatocellular damage, evidenced by elevated liver enzymes (AST, ALT, ALP) and histopathological alterations.
  • Both APAP and APAP + ozone groups showed significantly higher liver enzyme levels compared to the control group.
  • Mice receiving ozone therapy exhibited significantly lower ALT and AST levels and reduced necrosis and inflammation compared to the APAP-only group.

Conclusions:

  • Medical ozone therapy demonstrated statistically significant beneficial effects in mitigating APAP-induced hepatotoxicity.
  • Ozone's known antioxidant properties likely contribute to its protective effects against liver injury.
  • The findings suggest that ozone therapy could be a valuable supplementary treatment for acute acetaminophen-induced liver damage.
Keywords:
AcetaminophenHepatotoxicityOzone

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