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Updated: Feb 14, 2026

Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
Published on: March 10, 2016
Primitive Endoderm Differentiation: From Specification to Epithelialization
Cécilia Bassalert1, Lorena Valverde-Estrella1, Claire Chazaud1
1GReD, Université Clermont Auvergne, CNRS, INSERM, Clermont-Ferrand, France.
Abstract:
At the time of implantation, the mouse blastocyst has developed three cell lineages: the epiblast (Epi), the primitive endoderm (PrE), and the trophectoderm (TE). The PrE and TE are extraembryonic tissues but their interactions with the Epi are critical to sustain embryonic growth, as well as to pattern the embryo. We review here the cellular and molecular events that lead to the production of PrE and Epi lineages and discuss the different hypotheses that are proposed for the induction of these cell types. In the second part, we report the current knowledge about the epithelialization of the PrE.
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