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Updated: Feb 14, 2026

High-Resolution Respirometry to Assess Bioenergetics in Cells and Tissues Using Chamber- and Plate-Based Respirometers
Published on: October 26, 2021
Assessing Mitochondrial Bioenergetics by Respirometry in Cells or Isolated Organelles
Guillaume Vial1,2, Bruno Guigas3,4
1INSERM U1042, Laboratoire Hypoxie-Physiopathologies cardiovasculaires et respiratoires HP2, Faculté de médecine et de pharmacie, Domaine de la merci, La Tronche, France.
Abstract:
Mitochondrial oxidative phosphorylation is central for generating ATP and maintaining energy homeostasis in most eukaryotic cells. The ex vivo measurement of mitochondrial oxygen consumption rates in intact cells or isolated organelles is a valuable approach to assess mitochondrial bioenergetics in various experimental conditions. In this chapter, we describe several step-by-step protocols for measuring mitochondrial respiration in intact cells, permeabilized cells (in situ mitochondria), and isolated organelles using both Clark-type polarographic oxygen electrode devices and the newly developed oxygen-sensing fluorophore-based Seahorse technology.
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