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Updated: Feb 14, 2026

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Post-Transcriptional Control of Angiotensin II Type 1 Receptor Regulates Osteosarcoma Cell Death

Yue Zhao1, Kaicheng Xu2, Peng Liu3

  • 1Department of Vascular Surgery, China-Japan Union Hospital, Jilin University, Changchun, China.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|February 27, 2018
PubMed
Abstract

Insights

MicroRNA-1248 (miR-1248) is elevated in osteosarcoma (OS) and reduces chemotherapy effectiveness by inhibiting AGTR1, suggesting miR-1248 suppression could improve patient outcomes.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are crucial in osteosarcoma (OS) tumorigenesis.
  • The role of miR-1248 in OS chemoresistance remains uninvestigated.

Purpose of the Study:

  • To investigate the effect of miR-1248 on the chemo-resistant potential of osteosarcoma.
  • To explore the relationship between miR-1248 and angiotensin II type 1 receptor (AGTR1) in OS.

Main Methods:

  • Quantified miR-1248 and AGTR1 levels in OS tissues using RT-qPCR and Western blotting.
  • Assessed the correlation between miR-1248 and AGTR1, and patient survival.
  • Utilized bioinformatics, dual luciferase reporter assays, CCK-8, and apoptosis assays to determine functional interactions.

Main Results:

  • miR-1248 was upregulated, while AGTR1 was downregulated in OS tissues.
  • High miR-1248 and low AGTR1 levels correlated with poorer patient survival.
  • miR-1248 directly inhibited AGTR1 translation and suppressed AGTR1-mediated apoptosis.

Conclusions:

  • Increased miR-1248 expression in OS may reduce chemotherapy efficacy by inhibiting AGTR1-mediated cell death.
  • Suppression of miR-1248 in OS cells could potentially enhance chemotherapy outcomes.
Keywords:
Angiotensin II type 1 receptor (AGTR1)ChemotherapyMiR-1248Osteosarcoma (OS)

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