Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Studies I: Kidney, Ureter, and Bladder Studies01:28

Imaging Studies I: Kidney, Ureter, and Bladder Studies

385
Kidney, Ureter, and Bladder (KUB) StudiesKidney, Ureter, and Bladder (KUB) studies are standard diagnostic imaging procedures used to assess the anatomy of the urinary system. They are commonly utilized for patients experiencing abdominal pain or urinary symptoms. By using a simple X-ray of the abdomen, KUB studies can reveal structural and pathological abnormalities within the kidneys, ureters, and bladder. These studies are particularly valuable in diagnosing kidney stones, urinary...
385
Relative Risk01:12

Relative Risk

2.2K
Relative risk (RR) is a statistical measure commonly used in epidemiology to compare the likelihood of a particular event occurring between two groups. This metric is important for evaluating the relationship between exposure to a specific risk factor and the probability of a particular outcome. It plays a crucial role in medical research, public health studies, and risk assessment. Relative risk quantifies how much more (or less) likely an event is to occur in an exposed group compared to an...
2.2K
Urinary Bladder01:23

Urinary Bladder

3.5K
The urinary bladder is a hollow, muscular sac that temporarily stores urine before it is expelled from the body. It can hold approximately 600 mL of urine prior to micturition. The bladder is retroperitoneal and located behind the pubic symphysis in the pelvic floor.
In males, the bladder is situated in front of the rectum, while in females, it is positioned anterior to the vagina and uterus. The bladder floor contains an inverted triangular area called the trigone, defined by the two ureteric...
3.5K
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

6.6K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
6.6K
Factors Affecting the Risk of Infection01:26

Factors Affecting the Risk of Infection

13.9K
The hosts' susceptibility to infection depends on several factors. The integrity of the skin and mucous membranes helps protect the body against microbial attacks. When the skin is altered, the chance of infection, limb loss, and even death increases.
The integrity and count of the white blood cells help the body resist pathogens and fight infection. When impaired, it reduces the body's resistance to pathogens. The acidic pH levels of the gastrointestinal, genitourinary tracts, and skin...
13.9K
Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

644
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
644

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[The safety and effectiveness of once daily detemir in patients with type 2 diabetes previously failing oral agents: the Chinese cohort from SOLVE(TM) observational study].

Zhonghua nei ke za zhi·2013
Same author

Assessment of carotid atherosclerosis in type 2 diabetes mellitus patients with microalbuminuria by high-frequency ultrasonography.

International journal of endocrinology·2013
Same author

[The baseline characteristics of patients with type 2 diabetes initiating insulin detemir : the Chinese cohort from the SOLVE(TM) study].

Zhonghua nei ke za zhi·2013
Same author

Assessment of carotid arterial wall elasticity in type 2 diabetes mellitus patients with microalbuminuria by real-time ultrasound elastography.

International journal of endocrinology·2012
Same author

Complexes of tRNA and maturation enzymes: shaping up for translation.

Current opinion in structural biology·2007
Same author

[Effects of ST6Gal I antisense oligonucleotide-mediated gene silencing on cell adhesion and invasiveness of hela cells].

Zhonghua zhong liu za zhi [Chinese journal of oncology]·2007

Related Experiment Video

Updated: Feb 14, 2026

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
09:24

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Published on: September 13, 2018

9.5K

Pioglitazone Use and Risk of Bladder Cancer: an In Vitro Study.

Shao-Ling Yang1,2, Ji-Jiao Wang1,3, Ming Chen1,4

  • 1Department of Endocrinology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China.

International Journal of Medical Sciences
|February 28, 2018
PubMed
Summary

Pioglitazone (PIO) did not promote bladder cancer cell growth or malignant changes in normal urothelial cells. Long-term PIO treatment downregulated p53 and cyclin D1 in bladder cancer cells, suggesting potential benefits for diabetic patients with bladder cancer.

Keywords:
PPAR gammabladder cancerpioglitazone

More Related Videos

An Orthotopic Bladder Cancer Model for Gene Delivery Studies
07:48

An Orthotopic Bladder Cancer Model for Gene Delivery Studies

Published on: December 1, 2013

13.1K
Culture of Bladder Cancer Organoids as Precision Medicine Tools
08:39

Culture of Bladder Cancer Organoids as Precision Medicine Tools

Published on: December 28, 2021

5.5K

Related Experiment Videos

Last Updated: Feb 14, 2026

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
09:24

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Published on: September 13, 2018

9.5K
An Orthotopic Bladder Cancer Model for Gene Delivery Studies
07:48

An Orthotopic Bladder Cancer Model for Gene Delivery Studies

Published on: December 1, 2013

13.1K
Culture of Bladder Cancer Organoids as Precision Medicine Tools
08:39

Culture of Bladder Cancer Organoids as Precision Medicine Tools

Published on: December 28, 2021

5.5K

Area of Science:

  • Oncology
  • Pharmacology
  • Cell Biology

Background:

  • The potential link between pioglitazone (PIO), a PPAR-gamma agonist, and bladder cancer risk remains a subject of debate.
  • Understanding PIO's in vitro effects on normal and cancerous bladder cells is crucial for risk assessment.

Purpose of the Study:

  • To investigate the in vitro effects of pioglitazone (PIO) on normal urothelial transitional epithelium (NUTE) cells and bladder cancer (J82) cells.
  • To evaluate whether PIO influences cell proliferation, apoptosis, and key protein expressions related to cell cycle regulation and apoptosis.

Main Methods:

  • NUTE cells from Sprague-Dawley rats and J82 bladder cancer cells were exposed to varying concentrations and durations of PIO.
  • Cell proliferation assessed via MTT assay, apoptosis via flow cytometry.
  • Expression levels of p53, cyclin D1, Bcl-2, and Bax determined by qRT-PCR and Western blot analysis.

Main Results:

  • PIO induced morphological changes, inhibited proliferation, and promoted apoptosis in NUTE cells in a time- and dose-dependent manner, but not in J82 cells within 72 hours.
  • No significant changes in p53, cyclin D1, Bcl-2, or Bax were observed in either cell type after 72 hours of PIO treatment.
  • Interestingly, long-term PIO treatment (over 192 hours) significantly downregulated p53 and cyclin D1 protein levels in J82 cells, but not NUTE cells.

Conclusions:

  • Pioglitazone does not appear to promote malignant alterations in normal urothelial cells or stimulate proliferation in bladder cancer cells.
  • The observed downregulation of p53 and cyclin D1 in J82 cells after prolonged PIO exposure suggests a potential therapeutic role for PIO in diabetic patients with bladder cancer.