Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.7K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.7K
Cell-surface Signaling01:21

Cell-surface Signaling

54.8K
Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects.
54.8K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.6K
5.6K
Microtubule Associated Proteins (MAPs)01:42

Microtubule Associated Proteins (MAPs)

6.0K
Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
6.0K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

16.3K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
16.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integration of biochemical and pharmacological evaluation of Pistacia lentiscus leaf polysaccharides: evidence for anti-inflammatory and gastroprotective activity.

Inflammopharmacology·2026
Same author

Synthesis of 2-Aryl-4-aminoquinazolines: Design, Molecular Docking, and In Vitro Assessment of Antibacterial and Cytotoxic Potential.

International journal of molecular sciences·2026
Same author

Bioimaging With Fluorescent Nucleic-Acid Aptamers for the Specific Detection and Quantification of Pseudomonas aeruginosa Alone and in Heterogeneous Bacterial Populations.

MicrobiologyOpen·2025
Same author

Targeted isolation of new antibacterial sesquiterpene coumarins from ammoniacum (Ferula communis L.).

Fitoterapia·2025
Same author

Corrigendum to "Past mastering of metal transformation enabled physicians to increase their therapeutic potential" [J. Trace Elem. Med. Biol. 71 (2022) 126926].

Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)·2022
Same author

Past mastering of metal transformation enabled physicians to increase their therapeutic potential.

Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)·2022

Related Experiment Video

Updated: Feb 13, 2026

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes
14:43

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Published on: November 26, 2008

15.6K

Mapping Changes in Cell Surface Protein Expression Through Selective Labeling of Live Cells.

Pierre Fechter1

  • 1UMR7242 Biotechnologie et Signalisation Cellulaire, CNRS, Institut de Recherche de l'Ecole de Biotechnologie de Strasbourg, Université de Strasbourg, Illkirch-Graffenstaden, France. P.Fechter@unistra.fr.

Methods in Molecular Biology (Clifton, N.J.)
|February 28, 2018
PubMed
Summary

Non-coding RNAs (ncRNAs) help bacteria adapt to new environments by altering membrane composition. A new method uses fluorescent dyes to enrich low-abundance surface proteins for easier analysis.

Keywords:
2D gel electrophoresisCell labelingCyDyeSurface proteinsncRNAs

More Related Videos

Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
11:56

Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons

Published on: February 12, 2014

16.1K
Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
07:38

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Published on: May 17, 2010

23.7K

Related Experiment Videos

Last Updated: Feb 13, 2026

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes
14:43

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Published on: November 26, 2008

15.6K
Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
11:56

Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons

Published on: February 12, 2014

16.1K
Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
07:38

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Published on: May 17, 2010

23.7K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Non-coding RNAs (ncRNAs) are crucial for bacterial adaptation to environmental changes.
  • ncRNAs modulate bacterial membrane composition in response to environmental shifts.
  • Analyzing changes in low-abundance surface proteins is challenging due to extraction difficulties.

Purpose of the Study:

  • To develop a method for easy, reproducible, and specific enrichment of surface proteins from total bacterial protein extracts.
  • To facilitate the monitoring of surface protein expression changes during bacterial adaptation.

Main Methods:

  • Directly labeling surface proteins on living bacterial cells using fluorescent dyes.
  • Performing total protein extraction after surface protein labeling.
  • Separating the enriched surface proteins using 2D gel electrophoresis.

Main Results:

  • The described method allows for specific enrichment of surface proteins.
  • The technique is easy to perform and yields reproducible results.
  • This facilitates the study of low-abundance surface proteins.

Conclusions:

  • The developed method simplifies the analysis of bacterial surface proteins.
  • This approach aids in understanding bacterial adaptation mechanisms mediated by ncRNAs.
  • Improved surface protein analysis contributes to the study of bacterial environmental responses.