3D Jet Writing: Functional Microtissues Based on Tessellated Scaffold Architectures.
Jacob H Jordahl1, Luis Solorio1, Hongli Sun1
1Biointerfaces Institute, NCRC B10-A175, 2800 Plymouth Rd, Ann Arbor, MI, 48109, USA.
Advanced Materials (Deerfield Beach, Fla.)
|February 28, 2018
Summary
3D jet writing creates precise, scalable scaffolds that mimic tissues. These advanced materials promote stem cell growth and bone healing in vivo without growth factors, showing promise for regenerative medicine and cancer research.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Adaptive manufacturing techniques are crucial for developing cell-instructive materials that mimic biological tissues.
- Current methods face limitations in precision, resolution, and scalability for complex tissue engineering applications.
Purpose of the Study:
- To introduce and characterize 3D jet writing, a novel adaptive manufacturing technique.
- To evaluate the potential of 3D jet writing scaffolds for stem cell applications and in vivo tissue regeneration.
- To explore the utility of these scaffolds as a platform for cancer cell metastasis research.
Main Methods:
- 3D jet writing, a modified electrospinning process, was employed to fabricate scaffolds with customizable pore geometries and large-scale capabilities.
- Human mesenchymal stem cells were cultured on the scaffolds to assess their expansion and differentiation potential in vitro.
- The constructs were implanted in vivo to evaluate their efficacy in healing critical bone defects without exogenous growth factors.
- The scaffolds were utilized as metastatic target sites in mice to observe cancer cell homing behavior.
Main Results:
- 3D jet writing achieved unprecedented precision and resolution in scaffold fabrication, with scalable production.
- Scaffolds successfully supported the 3D expansion and differentiation of human mesenchymal stem cells.
- Implantation of constructs resulted in the healing of critical bone defects in vivo, independent of growth factors.
- Circulating cancer cells demonstrated homing to the simulated osteogenic environment on the scaffolds, even in abnormal anatomical locations.
- Tessellated microtissues were formed, highlighting the platform's versatility.
Conclusions:
- 3D jet writing is a powerful adaptive manufacturing technique for creating advanced biomaterial scaffolds.
- These scaffolds hold significant potential for regenerative medicine, particularly in bone defect repair and stem cell applications.
- The technology offers a versatile 3D cell culture platform applicable to cancer biology and stem cell biotechnology research.
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