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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Two new bis-C20-diterpenoid alkaloids with anti-inflammation activity from Aconitum bulleyanum
Xiao-Yan Duan1, Da-Ke Zhao2, Yong Shen1
1a College of Agriculture and Biotechnology , Yunnan Agricultural University , Kunming 650201 , China.
Abstract:
Two new bis-C20-diterpenoid alkaloids, bulleyanines A and B (1 and 2), were isolated from Aconitum bulleyanum. Their structures were elucidated by comprehensive spectroscopic analyses including UV, IR, MS, 1D and 2D NMR. Biological activity tests indicated that compound 1 exhibited significant inhibitory activity against nitric oxide (NO) production in lipopolysaccharide (LPS)-activated RAW264.7 macrophages with the inhibition rate of 74.60% (40 μmol/L), while positive control dexamethasone gave 78.70% inhibition at 100 μg/ml.
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