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Published on: June 2, 2023
Fibrinolytic Protease from Marine Streptomyces rubiginosus VITPSS1
Priyanka Verma1, Shruti Chatterjee1, Merlyn S Keziah1
1School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India.
Introduction:
Fibrinolytic enzymes must currently originate a significant product in the arena of medical research. Very limited studies are stated on fibrinolyticenzyme production from actinomycetes.
Methods:
Streptomyces sp. isolated from marine soil was chosen to optimize its fibrinolytic protease production.16s rRNA sequencing confirmed the isolated potent strain to be Streptomyces rubiginosus VITPSS1. A fibrinolytic protease was then purified from Streptomyces rubiginosus VITPSS1, with the target of producing a cost effective feasible enzyme from a potential actinomycete.
Results:
SDS-PAGE results exhibited a protein band of about 45 kDa and the fibrinolytic band was detected by zymography. Optimization of physical and nutritional parameters for fibrinolytic protease production from a marine soil isolate Streptomyces strain was done by response surface methodology. The optimal cultural condition for fibrinolytic protease production was obtained with response surface methodology was based on OFAT results, it was inferred that glycerol, Soyabean meal, pH 7.2 and temperature 37°C. The optimization of the production of fibrinolytic protease with response surface methodology bring about two-folds increase in production by Streptomyces rubiginosus VITPSS1.
Conclusion:
Thus, this study presents its novelty by highlighting the potential of marine Streptomyces as a significant source for fibrinolytic enzyme production.
Insights
Marine actinomycetes, specifically Streptomyces rubiginosus VITPSS1, show potential for producing fibrinolytic enzymes. Optimization significantly increased enzyme yield, highlighting their value in medical research.
Area of Science:
- Microbiology
- Biochemistry
- Enzymology
Background:
- Fibrinolytic enzymes are crucial in medical research, yet their production from actinomycetes remains underexplored.
- Limited studies focus on fibrinolytic enzyme generation by actinomycetes, indicating a research gap.
Purpose of the Study:
- To optimize fibrinolytic protease production from a marine actinomycete isolate.
- To identify cost-effective methods for producing fibrinolytic enzymes from potential actinomycetes.
Main Methods:
- Isolated and identified a potent fibrinolytic strain from marine soil as Streptomyces rubiginosus VITPSS1 using 16S rRNA sequencing.
- Purified the fibrinolytic protease and optimized production parameters (glycerol, soybean meal, pH 7.2, 37°C) using response surface methodology.
Main Results:
- SDS-PAGE revealed a 45 kDa protein band, confirmed by zymography.
- Optimization using response surface methodology led to a two-fold increase in fibrinolytic protease production.
- Optimal conditions included glycerol, soybean meal, pH 7.2, and 37°C.
Conclusions:
- Marine Streptomyces, exemplified by S. rubiginosus VITPSS1, represent a promising source for fibrinolytic enzyme production.
- This study demonstrates a novel approach to enhance enzyme yield through optimized cultivation conditions.
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