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CD64 on monocytes and granulocytes in severe acute bronchiolitis: Pilot study on its usefulness as a bacterial

Alberto García-Salido1, Ana Serrano-González1, Juan Casado-Flores1

  • 1Pediatric Critical Care Unit, Hospital Infantil Universitario Niño Jesús, Madrid, Spain.

Journal of Leukocyte Biology
|February 28, 2018
PubMed

Insights

CD64 receptor expression was higher in children with severe acute bronchiolitis and probable bacterial infection. This finding suggests CD64 may serve as a potential biomarker for bacterial infections in pediatric critical care settings.

Area of Science:

  • Immunology
  • Pediatric Infectious Diseases

Background:

  • The CD64 receptor is a known biomarker for bacterial infections.
  • Severe acute bronchiolitis (SAB) in children can be challenging to differentiate from bacterial infections.

Purpose of the Study:

  • To investigate CD64 surface expression on monocytes and granulocytes in children with SAB.
  • To determine if CD64 expression correlates with probable bacterial infection (PBI) markers in SAB.

Main Methods:

  • Prospective observational pilot study involving 32 children admitted to pediatric critical care.
  • CD64 expression measured by flow cytometry within 24 hours of admission.
  • Correlation analysis with traditional PBI biomarkers (procalcitonin, C-reactive protein, leukocyte count, radiographic findings).

Main Results:

  • CD64 surface expression was significantly higher in children with PBI (AUC=0.73, P=0.042).
  • The percentage of CD64-positive granulocytes was elevated in children with PBI.
  • A correlation was observed between CD64 expression and established PBI criteria.

Conclusions:

  • CD64 surface expression on monocytes and granulocytes is higher in children with SAB and PBI.
  • CD64 shows potential as a biomarker for bacterial infections in this pediatric population.
  • Further large-scale clinical studies are required to confirm the diagnostic accuracy of CD64.

Keywords:
CD64acute bronchiolitisbacterial infectionsflow cytometryrespiratory syncytial virus

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