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Organellar transcriptome sequencing reveals mitochondrial localization of nuclear encoded transcripts
Ankit Sabharwal1, Disha Sharma2, Shamsudheen Karuthedath Vellarikkal1
1Genomics and Molecular Medicine, CSIR Institute of Genomics and Integrative Biology (CSIR-IGIB), Mathura Road, Delhi 110 020, India; Academy of Scientific and Innovative Research (AcSIR), CSIR IGIB South Campus, Mathura Road, Delhi 110020, India.
Mitochondrion
|February 28, 2018
Summary
Researchers identified nuclear-encoded transcripts that localize to mitochondria in zebrafish. This finding reveals a new mechanism for mitochondrial-nuclear communication and influences mitochondrial function.
Area of Science:
- Cell Biology
- Genomics
- Mitochondrial Biology
Background:
- Mitochondria perform diverse cellular functions beyond ATP production.
- Mitochondrial function relies on both mitochondrial and nuclear genomes.
- Nuclear-encoded transcripts localizing to mitochondria are poorly understood.
Purpose of the Study:
- To identify nuclear-encoded transcripts that localize to the mitochondrial matrix.
- To investigate the role of these transcripts in mitochondrial-nuclear crosstalk.
Main Methods:
- Deep sequencing approach in a zebrafish model.
- Prioritization of candidate transcripts.
- Experimental validation of transcript localization.
Main Results:
- Identified 292 candidate nuclear-encoded transcripts potentially localizing to the mitochondrial matrix.
- Experimentally confirmed the mitochondrial localization of the transcript for nuclear-encoded ribosomal protein 11 (Rpl11).
Conclusions:
- Comprehensive analysis of nuclear-encoded transcript localization to mitochondria.
- Revealed a new layer of biomolecular pathways in mitochondrial-nuclear crosstalk.
- Provides a foundation for understanding the influence of these transcripts on mitochondrial function.
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