Molecular characterization of complement component 3 (C3) in Mytilus coruscus improves our understanding of bivalve...

Complement component 3 (C3) plays a central role in the complement system whose activation is essential for all the important functions performed by this system. Here, a novel C3 gene, termed Mc-C3, was identified from thick shell mussel (Mytilus coruscus). The deduced Mc-C3 protein possessed the characteristic structure features present in its homologs and contained the A2M_N_2, ANATO, A2M, A2M_comp, A2M_recep, and C345C domains, as well as the C3 convertase cleavage site, thioester motif, and conserved Cys, His, and Glu residues. Mc-C3 gene constitutively expressed in all examined tissues and predominantly expressed in immune-related tissues such as gills, hemocytes and hepatopancreas. After stimulation with lipopolysaccharide or Cu²⁺, the expression of Mc-C3 was significantly induced in gills. Further luciferase reporter assays showed the ability for activation of NF-κB signaling transduction of Mc-C3a. Taken together, these results show that C3 may play an essential role in the immune defense of M. coruscus. The present data therefore provide a more detailed insight into the functional activities of the bivalve complement system.