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Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains
Published on: April 27, 2018
Integrative whole-brain neuroscience in larval zebrafish
Gilles C Vanwalleghem1, Misha B Ahrens2, Ethan K Scott3
1School of Biomedical Sciences, The University of Queensland, St. Lucia, QLD 4072, Australia.
Abstract:
Due to their small size and transparency, zebrafish larvae are amenable to a range of fluorescence microscopy techniques. With the development of sensitive genetically encoded calcium indicators, this has extended to the whole-brain imaging of neural activity with cellular resolution. This technique has been used to study brain-wide population dynamics accompanying sensory processing and sensorimotor transformations, and has spurred the development of innovative closed-loop behavioral paradigms in which stimulus-response relationships can be studied. More recently, microscopes have been developed that allow whole-brain calcium imaging in freely swimming and behaving larvae. In this review, we highlight the technologies underlying whole-brain functional imaging in zebrafish, provide examples of the sensory and motor processes that have been studied with this technique, and discuss the need to merge data from whole-brain functional imaging studies with neurochemical and anatomical information to develop holistic models of functional neural circuits.
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