Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evidence for functional differences between two flagellar dynein ATPases.

S M Penningroth, D D Peterson

    Cell Motility and the Cytoskeleton
    |January 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Potential novel predictors of mortality in end-stage renal disease patients with sleep disorders.

    American journal of kidney diseases : the official journal of the National Kidney Foundation·2000
    Same author

    A preliminary study of the effects of correction of anemia with recombinant human erythropoietin therapy on sleep, sleep disorders, and daytime sleepiness in hemodialysis patients (The SLEEPO study).

    American journal of kidney diseases : the official journal of the National Kidney Foundation·1999
    Same author

    Longitudinal and cross-sectional analysis of HEALTH WATCH data with a subset of perimenopausal women and matched controls.

    The journals of gerontology. Series A, Biological sciences and medical sciences·1999
    Same author

    Nocturia. A rarely recognized symptom of sleep apnea and other occult sleep disorders.

    Archives of internal medicine·1996
    Same author

    Transient ischemic attacks and minor stroke during sleep. Relationship to obstructive sleep apnea syndrome.

    Stroke·1995
    Same author

    Night terrors in an adult precipitated by sleep apnea.

    Sleep·1995

    Flagellar motility in sea urchin sperm is powered by two distinct dynein ATPases: high-affinity dynein ATPase (HADA) and low-affinity dynein ATPase (LADA). Both are movement-coupled and likely control force generation and beat frequency, respectively.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Biophysics

    Background:

    • Flagellar motility is crucial for sperm function and requires efficient energy coupling.
    • Axonemal dyneins are motor proteins responsible for flagellar movement, utilizing ATP hydrolysis.
    • Understanding the distinct roles of different dynein populations is key to deciphering flagellar mechanics.

    Purpose of the Study:

    • To investigate the energy coupling mechanisms in flagellar motility.
    • To differentiate and characterize the ATPase activities of axonemal dyneins.
    • To determine the roles of distinct dynein classes in force generation and beat frequency.

    Main Methods:

    • Utilized demembranated, reactivated sea urchin spermatozoa (Arbacia punctulata).
    • Measured ATP-dependence of ATPase activity across a range of ATP concentrations (4–600 μM).

    Related Experiment Videos

  • Employed Eadie-Scatchard plot analysis to identify and quantify dynein ATPase activities.

    • Main Results:

    • Identified two distinct axonemal dynein ATPases: high-affinity dynein ATPase (HADA) and low-affinity dynein ATPase (LADA), with apparent Michaelis constants of 4 μM and 161 μM ATP, respectively.
    • Both HADA and LADA were found to be 65% movement-coupled.
    • Movement-coupled HADA and LADA exhibited increased Michaelis constants (12 μM and 271 μM ATP), suggesting altered function during movement.

    Conclusions:

    • Flagellar force generation is likely controlled by movement-coupled HADA.
    • Flagellar beat frequency is potentially regulated by movement-coupled LADA.
    • Mechanochemical activity in flagellar motility involves at least two distinct, functionally specialized classes of axonemal dyneins.