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Updated: Feb 13, 2026

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
09:58

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Published on: May 2, 2017

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Downregulation of SETD8 by miR-382 is involved in glioma progression

Zhiming Ma1

  • 1Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, PR China.

Pathology, Research and Practice
|March 1, 2018
PubMed
Abstract

Insights

SETD8 (SET domain-containing protein 8) promotes glioma progression by increasing cell proliferation and migration. MicroRNA-382 (miR-382) directly targets and downregulates SETD8, suggesting a potential therapeutic strategy for glioma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • SETD8 (SET domain-containing protein 8) is implicated in various biological processes, including carcinogenesis.
  • The specific role of SETD8 in glioma progression remains largely uninvestigated.

Purpose of the Study:

  • To investigate the role of SETD8 in glioma progression.
  • To explore the relationship between SETD8 and microRNA-382 (miR-382) in glioma.

Main Methods:

  • Quantitative PCR (qPCR) and Western blot to assess SETD8 and miR-382 expression.
  • MTT and wound healing assays to evaluate cell proliferation and migration.
  • Luciferase assays to validate miR-382 targeting of SETD8.

Main Results:

  • SETD8 expression is significantly upregulated in glioma tissues and cells compared to normal controls.
  • SETD8 enhances glioma cell proliferation and migration in vitro and in vivo.
  • miR-382 directly targets SETD8, and an inverse correlation exists between miR-382 and SETD8 levels in glioma patients.

Conclusions:

  • Oncogenic SETD8 is regulated by miR-382 and plays a crucial role in glioma progression.
  • The miR-382/SETD8 axis presents potential therapeutic targets for glioma treatment.
Keywords:
GliomaMigrationProliferationSETD8miR-382

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